lair1 (Jackson Laboratory)
86
Structured Review
Jackson Laboratory
lair1
Lair1, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lair1/pmc12890506-196-3-6?v=Jackson+Laboratory
Average 86 stars, based on 1 article reviews
Lair1, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lair1/pmc12890506-196-3-6?v=Jackson+Laboratory
Average 86 stars, based on 1 article reviews
lair1 - by Bioz Stars,
2026-07
86/100 stars
Images
1) Product Images from "LAIR1 prevents excess inflammatory tissue damage in Staphylococcus aureus skin infection and cutaneous T cell lymphoma"
Article Title: LAIR1 prevents excess inflammatory tissue damage in Staphylococcus aureus skin infection and cutaneous T cell lymphoma
Journal: JCI Insight
doi: 10.1172/jci.insight.183935
Figure Legend Snippet: ( A – C ) CD4 + cells were isolated from CTCL patient peripheral blood and analyzed by single cell RNA-Seq. UMAP projections show 92,496 mononuclear cells from 16 peripheral blood samples from 6 patients with CTCL across multiple time points. ( A ) Cell type assignment by highest spearman rho of purified immune populations in the Human Primary Cell Atlas. ( B and C ) UMAP plots show expression of LAIR1 ( B ) and LAIR2 ( C ). ( D ) Illustration depicts hypothesis that LAIR2 overexpression reduces LAIR1 signaling in CTCL. ( E ) Illustration depicts mouse model for loss of LAIR1 signaling via KO of Lair1 .
Techniques Used: Isolation, Single Cell, RNA Sequencing, Purification, Expressing, Over Expression
![WT and Lair1- KO mice were infected s.c. with 1 × 10 7 CFU S . aureus USA300 LAC. ( A and B ) Lesions were monitored over 14 days and quantified as abscess ( A ) and dermonecrosis ( B ) area (noninvasive measurements, area calculated: A = ([pi/2] × l × w). Statistical analysis by repeated measures 2-way ANOVA with Bonferroni post hoc tests. Results are representative of 5 independent experiments ( n = 40 WT, n = 40 Lair1 KO). ( C ) Representative gross images of S . aureus skin lesions in WT (left) and Lair1 -KO (right) mice on 4dpi. Abscess boundaries are indicated with the dashed lines. Dermonecrosis is the central erythematous lesion. ( D ) Representative H&E stains of lesions at 2 dpi. Scale bars: 1 mm. Asterisks indicate abscesses, and solid lines indicate areas of dermonecrosis, which are larger in Lair1 KO.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_0506/pmc12890506/pmc12890506__jciinsight-11-183935-g244.jpg "WT and Lair1- KO mice were infected s.c. with 1 × ...")
Figure Legend Snippet: WT and Lair1- KO mice were infected s.c. with 1 × 10 7 CFU S . aureus USA300 LAC. ( A and B ) Lesions were monitored over 14 days and quantified as abscess ( A ) and dermonecrosis ( B ) area (noninvasive measurements, area calculated: A = ([pi/2] × l × w). Statistical analysis by repeated measures 2-way ANOVA with Bonferroni post hoc tests. Results are representative of 5 independent experiments ( n = 40 WT, n = 40 Lair1 KO). ( C ) Representative gross images of S . aureus skin lesions in WT (left) and Lair1 -KO (right) mice on 4dpi. Abscess boundaries are indicated with the dashed lines. Dermonecrosis is the central erythematous lesion. ( D ) Representative H&E stains of lesions at 2 dpi. Scale bars: 1 mm. Asterisks indicate abscesses, and solid lines indicate areas of dermonecrosis, which are larger in Lair1 KO.
Techniques Used: Infection
Figure Legend Snippet: ( A – F ) Skin lesions were collected at 18 hours after infection, homogenized, and cytokine production measured by multiplex cytokine array. ( A ) Heatmap shows average WT and Lair1 KO z -scored cytokine concentrations. ( B – F ) Bar graphs show all cytokines with significantly different expression between WT and Lair1 KO. ( G – L ) BMDMs were generated in vitro and treated with PBS or S . aureus at a multiplicity of infection (MOI) of 10:1 for 18 hours. Cell supernatant was analyzed by multiplex cytokine array. ( G ) Heatmap shows average WT and Lair1- KO cytokine concentrations in PBS and S . aureus groups. ( H – L ) Bar graphs show all cytokines with significantly different expression between WT and Lair1 KO ( n = 2 independent infections, 2–6 mice per group). ( B – F and G – L ) Two-way ANOVA with Fisher’s LSD for multiple comparisons. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.
Techniques Used: Infection, Multiplex Assay, Expressing, Generated, In Vitro
Figure Legend Snippet: ( A and C ) BMDM from WT and Lair1-KO mice were treated with PBS or PAM3CSK4 for 6 hours and were then subjected to RNA-Seq. ( B and D ) Skin punch biopsies from WT and Lair1 -KO mouse S . aureus skin infections were taken at 2 dpi and were then subjected to RNA-Seq. GSEA was used to define the top 20 mouse Reactome pathways with enrichment scores and nominal P value in genes upregulated in Lair1 KO in BMDM treated with PAM3CSK4 ( A ) and 2 dpi SSTI ( B ); collagen-related pathways common to both analyses are bolded in teal. Genes from the 4 pathways, many of which overlapped, were classified into 3 general categories: collagen genes, collagen synthesis genes, and extracellular matrix (ECM) remodeling genes. Heatmaps show z -scored expression for these genes for BMDM ( C ) and S . aureus SSTI ( D ). ( E and F ) BMDM from WT and Lair1 -KO mice were treated with PBS or PAM3CSK4 for 24 hours and were then fixed and stained for Serpin H1. Representative images from 3 independent experiments are shown ( E ). Scale bars: 50 μm. Images were analyzed in CellProfiler and mean Serpin H1 cytoplasmic fluorescence intensity per cell was calculated ( F ). Mean ± SEM are indicated by black bars. Statistical analysis was done by 2-way ANOVA with multiple comparisons.
Techniques Used: RNA Sequencing, Expressing, Staining, Fluorescence
Figure Legend Snippet: WT and Lair1- KO neutrophils were isolated from mouse bone marrow. ( A ) Amplex red fluorescence measured by plate reader indicates hydrogen peroxide (H 2 O 2 ) production in neutrophils treated with PBS or S . aureus at MOI 50:1 for 30 minutes. ( B ) Ratio of WT/KO lysosome probe MFI measured by flow cytometry indicates lysosome acidification in neutrophils treated with PBS or S . aureus at MOI 100:1 for 30 minutes. ( C ) Neutrophils incubated for 30 minutes with PBS or S . aureus bioparticles (pHrodo) that fluoresce when acidified in the lysosome were subjected to flow cytometry. ( D ) Neutrophils incubated for 30 minutes with PBS or mCherry-expressing S . aureus at MOI 50:1 were subjected to flow cytometry. mCherry MFI is shown as a ratio of KO to WT. Statistical analysis for all panels was done by 2-way ANOVA with Fisher’s LSD. ** P < 0.01.
Techniques Used: Isolation, Fluorescence, Flow Cytometry, Incubation, Expressing
